asta (Developmental Studies Hybridoma Bank)
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Developmental Studies Hybridoma Bank
asta
Asta, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/asta/anti-allatostatin/bio_rxiv__2025__06__30__662346-223-24-25
Average 93 stars, based on 20 article reviews
Asta, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/asta/anti-allatostatin/bio_rxiv__2025__06__30__662346-223-24-25
Average 93 stars, based on 20 article reviews
asta - by Bioz Stars,
2026-09
93/100 stars
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1) Product Images from "Metabolic control of enteroendocrine cell fate through a redox state sensor CtBP"
Article Title: Metabolic control of enteroendocrine cell fate through a redox state sensor CtBP
Journal: bioRxiv
doi: 10.1101/2025.06.30.662346
Figure Legend Snippet: (A) ctbp deficiency ( CtBP 03463 /CtBP 87De-10 ) in Drosophila larvae results in a reduction on relative number of EE cells on HSD (30% sucrose), quantified in (B) . The data were compared with unpaired t-test, P = 0.0012. (C) Upper panel: The distribution of Dh31-, Tk-, and AstA-expressing EE cells in Drosophila larvae: a schematic overview. Lower panel: Whole-body ctbp loss-of-function ( CtBP 03463 /CtBP 87De-10 ) results in a loss of Dh31-, Tk-, and AstA-expressing EE cells on HSD. (D) Quantification of C. Two-tailed t-tests, *P < 0.01; **P = 0.0032. (E) Targeted loss of CtBP in EE cells (Pros-GAL4>CtBP RNAi) leads to reduced relative number of EE cells in larvae on HSD. Two-tailed t-test, P = 0.0046. (F) EE cell specific loss of CtBP function (Pros-GAL4 > CtBP RNAi ) on HSD reduces EE cell size (Mann-Whitney U test, P = 0.00313). (G) Loss of CtBP function in EE cells (Pros-GAL4>CtBP RNAi) leads to sugar intolerance of Drosophila larvae. The pupariation rate of larvae fed on HSD (20% sucrose) were compared with the log-rank test (χ = 88.6, P < 0.0001). (H) Intestinal lipid levels are reduced in larvae with EE-specific CtBP deficiency (Pros-GAL4>CtBP RNAi), quantified as relative Oil Red O intensity in (I) , unpaired t-test, **** P < 0.0001. (J) Loss of Dh31, Tk and AstA function in EE cells (Pros-GAL4> RNAi) leads to sugar intolerance of Drosophila larvae. The pupariation rate of larvae fed on HSD (20% sucrose) were compared using a log-rank test with the adjustment for multiple comparisons (χ = 59.4, P < 0.0001). ( K ) Intestinal lipid levels are reduced in larvae with EE-specific Dh31-deficiency (Pros-GAL4>Dh31 RNAi), quantified as relative Oil Red O intensity in ( L) , unpaired t-test, **** P < 0.0001.
Techniques Used: Expressing, Two Tailed Test, MANN-WHITNEY
Figure Legend Snippet: (A) The ratiometric SoNar and iNap sensors were expressed by Pros-GAL4 ts driver to monitor the NAD + /NADH and NADPH/NADP + ratios in EE cells. GAL4 expression was activated 16 h prior EE cell redox state measurements. EE cells of HSD fed larvae showed elevated reduction of NAD + and NADP + in EE cells when compared to those of 2-deoxyglucose (2-DG)-fed animals. The data were analyzed using the Mann-Whitney test, with ****P < 0.0001. (B) Feeding larvae with nicotinamide riboside (NR), a precursor of nicotinamide adenine dinucleotide, increases the proportion of EE cells (Pros + ) in the Drosophila intestine. Data were analyzed using an unpaired t-test (****P < 0.0001). NR feeding also increases the number of Dh31-positive cells, with statistical significance determined by unpaired t-tests (*P = 0.0149; ***P = 0.0008). (C) EE cell specific knockdown (Pros-GAL4) of Naprt , a critical enzyme in NAD biosynthesis, leads to sugar intolerance. Pupariation rates of control and experimental genotype on HSD were compared using the log-rank test (χ = 64.2, P < 0.0001). (D) EE cell specific knockdown (Pros-GAL4) of Naprt reduces relative EE cell numbers in the Drosophila intestine as well as (E) number of Dh31, AstA and Tk positive cells. The data were analyzed using unpaired t-test, *P < 0.03; ****P < 0.0001. (F) Transgenes expressing the bacterial enzymes LbNOX and TPNOX, which oxidize NADH and NADPH in EE cells, respectively, impair larval development on HSD. Pupariation rate is analyzed using the log-rank test, revealing significant effects for both LbNOX (χ = 43.8, P < 0.0001) and TPNOX (χ = 54.1, P < 0.0001).
Techniques Used: Expressing, MANN-WHITNEY, Knockdown, Control
Related Articles
Blocking Assay:Article Title: Metabolic control of enteroendocrine cell fate through a redox state sensor CtBP Article Snippet: .. Following blocking, the tissues were incubated with antibodies against Prospero (1:500, Developmental Studies Hybridoma Bank, MR1A), Mesh (1:1000, a kind gift from Mikio Furuse), AstA (Developmental Studies Hybridoma Bank, 5F10C), Dh31 or Tk (a kind gift from Jan Veenstra). .. Fluorescent secondary antibodies were obtained from Invitrogen (Thermo Fisher Scientific). Incubation:Article Title: Metabolic control of enteroendocrine cell fate through a redox state sensor CtBP Article Snippet: .. Following blocking, the tissues were incubated with antibodies against Prospero (1:500, Developmental Studies Hybridoma Bank, MR1A), Mesh (1:1000, a kind gift from Mikio Furuse), AstA (Developmental Studies Hybridoma Bank, 5F10C), Dh31 or Tk (a kind gift from Jan Veenstra). .. Fluorescent secondary antibodies were obtained from Invitrogen (Thermo Fisher Scientific). |